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Image Search Results
Journal: mBio
Article Title: Sp1 facilitates continued HSV-1 gene expression in the absence of key viral transactivators
doi: 10.1128/mbio.03479-23
Figure Lengend Snippet: Antibodies for immunoblot experiments
Article Snippet: ICP0 ,
Techniques: Western Blot
Journal:
Article Title: Evidence for Controlled Incorporation of Herpes Simplex Virus Type 1 UL26 Protease into Capsids
doi:
Figure Lengend Snippet: VP24 is present at similar levels in all forms of intracellular HSV-1 capsids. Western blots of A, B, and C capsids purified by sucrose gradient sedimentation from HSV-1 (KOS)-infected Vero cells are shown. Threefold dilutions of capsids were run in parallel. Replicate Western blots were probed with MAbs directed against VP5 and VP23 (upper panel), MAb MCA406, which recognizes VP21 and VP22a (center panel), and MAb 9-2, directed against VP24 (lower panel). Chemiluminescent Western blots were exposed to Kodak XAR-5 film, and exposures were prepared for presentation using a Kodak DCS400 digital camera and Adobe PhotoShop 5.0.
Article Snippet: Antibodies were added to blots for 2 h at the following dilutions: monoclonal antibody (MAb) 13-183 against VP5 (Advanced Biotechnologies Inc.) at 1:1,000; MAb 1D2 against VP23 ( 40 ) at 1:2,000;
Techniques: Western Blot, Purification, Sedimentation, Infection